comparative analysis of the in-vitro effects of various sterilization techniques and different disinfectants on the micropropagation of explants of Asparagus Racemosus

Asparagus racemosus belongs to the Asparagaceae family and genus is an endangered plant species that have been listed in red data book. For conventional breeding of racemosus, its tubers are used whichpossess a wide range antioxidants, leading wastage highly oxidative tuber roots. Using in-vitro propagation method we can propagate using nodal explants successfully only contamination-free environment. The sources contamination be present media or associated with explantswhich need eradicated various sterilization techniques disinfectants. This paper presents two different treatment disinfectant Sodium hypochlorite sterilize explants. was sterilized autoclave microwave oven explant sterilizing agent sodium three concentrations 5%, 10%, 15% for 5 min. were cultured MS basal medium supplemented 30g sucrose, as carbon source 7g agar, gelling agent. temperatureinside growth chamber maintained at 24±2°C photoperiod 16 h daylight 8 dark.